“Basils 2025” – Second Report

Summary 

Regarding the issue of the instability of the purple color in Ocimum basilicum 

Regarding the issue of the activation of the purple color in Ocimum tenuiflorum 

In the Basils, is it Linalool, or another Terpene cocktail, that certain species of fruiting calyxes-cutting ants are fond of?

This written contribution, in particular regarding the first two sections, has been induced by recent questions and comments from Noelle Fuller, in the USA, and Conrad Richters (the founder of Richters Seeds), in Canada, about the issue of color stability in Ocimum basilicum and Ocimum tenuiflorum. This is a topic I began to delve into, in the spring of 2025, and here are some of my observations gathered since then.

According to Conrad: «Light intensity, temperature, and micronutrient deficiencies are known to affect colour in purple basils, and those effects are known to be mediated by regulatory genes. It appears that anthocyanin gene expression is modified by these environmental factors, and it appears that these changes can be passed on to successive generations even if the genes themselves are still present. I would not be surprised if the situation in krishna tulsi is similar which may explain our mutual frustration with this Tulsi. »

Purple Basil “Red Genovese” from Richters Seeds, Canada

According to Noelle: «It looks like everything sold as Krishna tulsi now appears to be some type of hybrid. And when growing the seed there was variability in appearance between plants as well. Which makes me wonder if the genetics are stable… If you have any insight I would love to hear, as I developed a preference for the Krishna phenotype from my research and now can’t find the seeds in north america. »

Young plant of Ocimum tenuiflorum, type “Krishna”, with purple leaves, along with an “Elephant Head” Amaranth

Regarding the issue of the instability of the purple color in Ocimum basilicum

This year, I grew, in pots and in the ground, the following varieties of basil marketed as “purple”: “Purple Rubin”, “Osmin”, “Rosie”, “Opalescent”, “Aromatto”, in addition to the bicolored Turkish ecotype from GRIN/USDA, “PI 172997”, and two almost-purple specimens of the recent green cultivar from Rutgers, resistant to downy mildew, “Devotion”, crossed with a purple basil “X’”, in 2022. 

The temperature conditions we are experiencing this year, in our almost desert-like region of Spain, are as follows: up to 36°C during a period in June and then up to 41°C in August – temperatures dropping by 15°C on August 21. This summer of 2025, no cultivar of Purple Basil, or bicolored, remained purple. They all inevitably turned green – including the veins of the leaves for some. It is a dark greening, but a greening nonetheless.

Purple Basil “Purple Delight”

As a reminder, I saw no difference this year between the varieties “Purple Rubin”, “Osmin”, “Rosie” (and also “Purple Delight” in 2022) in terms of their purple coloration. At least, in the very early stages of cultivation, as plants of 30/40 cm started to lose purple color in their older leaves as early as the beginning of June – at least in this year 2025.

On the contrary, I did not observe such an extreme degradation of the purple color in the Basil plants during the summer of 2022, as my photographs from the end of July 2022 highlight a beautiful coloration for the “Purple Delight” cultivar (Richters Seeds), a little less for the “Osmin” cultivar (Kokopelli), while there was, however, total greening for the “Red Genovese” cultivar (Richters Seeds). I assume that the summer of 2022 was much less hot.

Purple Basil “Purple Delight”, end of July 2022

It would seem, therefore, a priori, that under identical solar lighting conditions, it is mainly temperature excesses that induce the Purple Basils to deactivate their anthocyanins and, thus, to lose their red-purple color.

Purple Basil “Purple Delight” on June 6th, 2025
Strong greening of purple leaves towards the end of June 2025

Anthocyanins give Basil plants their red, purple, and mauve colors. For the record, there are six main types of anthocyanidins: delphinidins, cyanidins, petunidins, pelargonidins, peonidins, and malvidins. These compounds induce the red, purple and mauve pigmentation of leaves, flowering tops, and other reproductive organs, as well as seeds.

According to a study from 1998, 14 different anthocyanins were isolated, including 11 cyanidin-based pigments and 3 peonidin-based pigments.  LINK.

The question arises as to why, with the increasing intensity of heat, and possibly light, the anthocyanins in purple leaves are destroyed, unlike those in flowering buds. Are they different anthocyanins? This is what the following two studies seem to suggest.

Thus, according to a study from 2019: «the study revealed that purple leaves mainly contain highly acylated anthocyanins, while purple flowers accumulate anthocyanins with low degree of decoration.». LINK.

Moreover, according to a study from 2020: «The analysis revealed a different composition in intact flowers (Figure 3b). Anthocyanins A and B were not detected, and anthocyanins C and D were only minor compounds. This seemed to be partially compensated by the accumulation of low acylated anthocyanins (i.e., cyanidin-3-Glc-5-(6-Mal)Glc and cyanidin malonyl-glucoside). Moreover, it was observed a higher concentration of cyanidin derivatives with low molecular weight as well as of quercetin derivatives, some of which were also accumulated in white flowers.». LINK

Reactivation of anthocyanins – and, therefore, of the purple color – in the Purple Basil “Osmin”, at the end of August 2025

Around August 30th, I noticed that green plants of the varieties “Osmin”, “Purple Rubin” (completely stripped of fruiting calices by the ants), and “Aromatto” have started to produce young leaves that are very purple again.

Reactivation of anthocyanins – and, therefore, of the purple color – in the Purple Basil “Osmin”, at the end of August 2025

On the other hand, a very beautiful plant of the “Purple Rubin” cultivar has remained completely green even though it is almost entirely hidden under very lush Mirabilis jalapa – that is, deprived of direct sunlight.

Partial reactivation of anthocyanins – and, therefore, of the purple color – in the Purple Basil “Purple Rubin”, at the end of August 2025

Conclusion. The Purple Basilics, or variegated with purple, tend – more or less quickly depending on the cultivars – to turn green, almost entirely, when the summer temperatures are too high for the stability of their anthocyanins. On the other hand, they regain their purple color as autumn approaches, as soon as the temperature drops drastically – while requiring direct sunlight (UV) that is essential for the reactivation of these anthocyanins.

Reactivation of anthocyanins – and, therefore, of the purple color – in the Purple Basil, “Purple Rubin”, at the end of August 2025

Regarding the issue of the activation of the purple color in Ocimum tenuiflorum

This year, I cultivated, in pots and in open ground, the following ecotypes of Ocimum tenuiflorum – formerly known as Ocimum sanctum: “Thai Green Tulsi” (Kokopelli), two accessions of “Krishna” (Kokopelli), a green ecotype from Cuba (GRIN/USDA. “PI 652057”) and a green ecotype from Gujarat in India (GRIN/USDA. “PI 288779”).

Some studies mention ecotypes of Ocimum tenuiflorum referred to as “intermediate”. Are these intra-specific crosses between the green and purple types? Have some “intermediate” ecotypes stabilized over the centuries?

First of all, let’s recall that according to the ad hoc scientific literature, the green ecotypes, called “Rama”, and the purple ecotypes, called “Krishna”, of Ocimum tenuiflorum, represent two different genetic entities – as highlighted by the following few studies.

1. “The complete chloroplast genome of Ocimum tenuiflorum L. subtype Krishna Tulsi and its phylogenetic analysis”. 2021. LINK. According to this study:

« The cp genome is 151,758 bp in length, including a large single copy (LSC) region of 82,794 bp, a small single-copy region (SSC) of 17,592 bp, and a pair of inverted repeated (IR) region of 25,686 bp. The cp genome of Krishna Tulsi encodes 129 genes, including 90 protein-coding, 31 transfer RNA (tRNA), and eight ribosomal RNA (rRNA) genes »

2. “The complete chloroplast genome of Ocimum tenuiflorum L. subtype Rama Tulsi and its phylogenetic analysis”. LINK. 2021. According to this study:

« The length of the complete circular chloroplast genome was 151,722 bp. It comprises an inverted repeat (IR) region with a repeat length of 25,677 bp, a large single-copy (LSC) region of 82,781 bp, and a small single-copy (SSC) region of 17,587 bp. The GC content of complete chloroplast genome, LSC, SSC, IR regions is 37.9%, 36.0%, 31.8%, and 43.1%, respectively. The chloroplast genome contains 134 genes, including 88 protein-coding genes, 38 transfer RNA genes, and eight ribosomal RNA genes.  »

3. “Molecular differentiation of the green and purple Tulsi (Ocimum tenuiflorum L.) and its application in authentication of market samples”. 2024. LINK. According to this study by researchers from Chennai, Tamil Nadu, India, green Tulsi was identified by two fragments (539 bp and 258 bp), and purple Tulsi was recognized by a single fragment (797 bp).

All that being said, my main discovery this summer of 2025, regarding the “Krishna” type of Ocimum tenuiflorum, concerns the activation of its purple color by direct sunlight at least, by certain spectrums such as ultraviolet light.

Indeed, this year, I grew my hundreds of Basil plants in pots in wall greenhouses sheltered under a roof of transparent polycarbonate sheets. Including, towards the end of June – and even more so because of the high temperatures at that time – the two types of “Krishna” that I had just received from Kokopelli. And as the seedlings were green – at 10 cm – I informed the seed sector that indeed, one of the seed producers had rightly raised the alert regarding the improper color.

I have, therefore, transferred, to the compost pile, the seedlings of the two types of “Krishna”, with the exception of a few, out of pure curiosity about their fate, which I transplanted into pots or into the garden, towards the end of July – alongside Swiss chard that I had also just replanted.

And I discovered that all the “Krishna” plants turned red in full sun except for two plants that remained green: one, 25 cm tall, in open pollination, while its pot was in the shade of two huge seed-bearing Basil plants, of the “Greek” and “Floral White Spire” cultivars, and the other, 35 cm tall, growing under the tent of insect-proof mesh partly shaded by olive trees.

Young plant of Ocimum tenuiflorum, type
“Krishna”, with its leaves in a process of anthocyanin-activation under the solar light

About five days after being exposed to direct sunlight, the leaves of the “Krishna” green plant began to take on purple hues.

In conclusion: it is direct sunlight, particularly its ultraviolet rays, that activates the purple/red color, and thus the anthocyanins, of the “Krishna” ecotypes of Ocimum tenuiflorum – including their so-called “intermediate” types.

Plants of the “Krishna” type, purple-red in color, will therefore turn green as soon as they are transferred to an area lacking ultraviolet light.

In the Basils, is it Linalool, or another Terpene cocktail, that certain species of fruiting calyxes-cutting ants are fond of?

In September 2022, in my monograph, “Les Qualités Extrêmement Médicinales de la Tulsi, Ocimum americanum”, (LINK) I wrote:

«And to finish on a naturalistic note! It is not just the perfumery industry that is obsessed with linalool: ants are too. Indeed, throughout the summer of 2022, I had to fight against columns of ants that systematically tore apart the flower stems – before the seeds were fully mature – only from varieties of Ocimum basilicum that possessed a “Linalool” chemotype. These columns of ants left behind, once their spoils were secured in the anthills, only strictly “are flower stems. They never targeted the ecotypes of Ocimum that had a “Citral”, “Camphor”, “Bisabolene”, “Cinnamon” or “Anise” chemotype. Only linalool motivated them. Linalool is known, among other medicinal activities, for its acaricidal, bactericidal, and fungicidal properties». 

And I asked, then, the following question: « Perhaps Linalool, in the ant nest, is the equivalent of Propolis in bee hives?» 

Stems shredded of their fruit calyces, by the Ants, in the Basil cultivar, “Obsession”  – with a chemotype Linalool

This summer 2025, once again, the ants started to shred the flower stems of the Basils – although later and only since mid-August. 

I observed and photographed them: they cut, with their jaws, the fruiting calyxes – whether with mature or immature seeds. Then, either they carry them down the plant, or they drop them so that other ants can take over.

At the end of August, in our garden, the “Philocimum” Ants have been very motivated for the following Basils: Ocimum campechianum and, for Ocimum basilicum, “Devotion”, “Obsession”, “Vert de Gènes a feuilles moyennes”, “PI 175793”, “PI 190100”, “White Spires”, “Osmin”, et “Purple Rubin” – but this is just the beginning of their harvest season…

… and, as the days went by, the ants then took hold of two “Siam Queen” plants, but only stripping the flower stems by 40%… and disappearing overnight…

… while others were attacking plants of the ecotype “PI 211586”.

Stems being stripped, by the Ants, of fruiting calyxes in the Basil cultivar, “Obsession”  – with a chemotype Linalool

Today, I am therefore asking the question of determining what the substance, or cocktail of substances, of their essential oil is, namely the common denominator among all these Basil plants, so that the ants are so fond of it. Is it Linalool?

Especially since there is information circulating, on the web, that Basil and Lavender are insect repellents… particularly for ants. This is due to the presence of Linalool. No joking? Furthermore, there are indeed blogs that explain how to get rid of ants on Basil!

In fact, according to a study from 2022, the two enantiomers of Linalool have distinct functions. Coriandrol primarily attracts pollinators, while Licareol appears to act as an insect repellent. LINK

It is very understandable, moreover, that Basil is associated with Linalool because the vast majority of Basil cultivars imposed on the general public, by the food industry, have a predominant chemotype of Linalool – sometimes up to 75% in their essential oil.

Notwithstanding, today, in light of my own gardening experience, it does not seem wise to assert that “Basil” is intrinsically a repellant for ants… due to the number of species, varieties, and ecotypes that characterize the genus Ocimum, and the extreme diversity of the chemotypes of their essential oils.

In order, therefore, to determine the substance of their essential oil, common to all these finely chopped basils by the foraging ants, I went in search of the relevant analyses – when they exist.

“Devotion”. This new acquisition is presented by Rutgers University as a type of “Classic Italian” Basil – thus with a Linalool chemotype.

“Obsession”. This new acquisition is presented by Rutgers University as a type of “Classic Italian” Basil – thus with a Linalool chemotype.

“Vert de Gènes a feuilles moyennes”.  This cultivar is caracterised by a Linalool chemotype.

PI 175793”. Originating from Çanakkale, Turkey, this ecotype is characterized by a chémotype Linalol – sometimes, up to 70%.

PI 190100”. Originating from Iran. Depending on the analyses, this ecotype is characterized by a Linalool content of about 10% and an Estragole content ranging from 10% to 65%.

I suspect that “PI 190100” is actually an Ocimum americanum var. pilosum, especially since a study characterizes it – in an analysis of supposed accessions of Ocimum basilicum – as having the highest citral content and the highest antioxidant activity. Indeed, its essential oil contains up to 26% citral and up to 12% β-citral. With such a third of Citral, or more, it is definitely a type of Basil known as “Lemon”.

“Osmin”. This purple cultivar is characterised by a chémotype Linalol – sometimes up to 65%. LINK 

“Purple Rubin”.This purple cultivar is characterised by a chémotype Linalol – sometimes up to 60/65%.

“Floral Spires White”. This cultivar, known as ornamental, is incorrectly presented on a plethora of commercial sites as a cultivar close to a Thai type with an aniseed scent – therefore, apparently, with an estragole chemotype.

I introduced it in 2014 at Kokopelli – with such a characterization that I copied from the Internet – and this is the first time I personally cultivate it this year. In reality, its growth habit has nothing to do with that of the Thai types – namely, Ocimum basilicum var. thyrsiflorum.

Indeed, the plants of “Floral Spires White” are characterized, strictly speaking, by a very compact and rounded shape, reminiscent of the dome of the “Greek” cultivar, sometimes called “Greco a Palla”. “Floral Spires White” reaches, in fact, 55 cm in diameter and 35 cm in height, while “Greek” reaches 45 cm in diameter and 30 cm in height.

Furthermore, like some ecotypes of Greek or Anatolian type, the foliage of “Floral Spires White” – with leaves of up to 5 cm in length, of which 1 cm is petiole – tends to turn to yellow with senescence, and seed maturity, in synergy with high solar intensity… whereas more shaded plants maintain green foliage.

PI 211586”. Based on the analyses, this ecotype is reported with a Linalool ratio ranging from 30% to 42% – as well as up to 36% Methyl Eugenol, 8% Citral, and high ratios of α-trans-bergamotene and epi-α-cadinol.

“Siam Queen”. It is difficult to find studies (and I refuse to pay for access to PDF of scientific articles) regarding the chemotype of this (alleged) strain introduced in 1997 – just as much, by the way, as for the chemotype of other Thai varieties, Ocimum basilicum var. thyrsiflorum. While it is clear that some Thai ecotypes are characterized by a high Estragol ratio, some studies also mention Linalool as a major component. LINK  LINK  LINK

In conclusion, after chemosystematic investigation focusing on the Basil species preferred, by the fruiting calyxes-cutter “Philocimum Ants”, it seems that the possibility of Linalool as a common denominator is the most plausible.